Protease Inhibitor:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Magnetic Beads:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Fluorescence:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
In Situ Hybridization:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Fluorescence In Situ Hybridization:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Immunohistochemistry:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
AST Assay:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Enzyme-linked Immunosorbent Assay:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
cDNA Synthesis:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
Real-time Polymerase Chain Reaction:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
RNA Sequencing:Article Title: SETDB1 promotes mammalian liver regeneration by stimulating cytokine CSF3 in hepatocytes.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER DMSO Sigma Cat# D2650 PEG300 MCE Cat# HY-Y0873 Tween-80 Sigma Cat# P4780 Hematoxylin buffer Maxim Cat# CTS-1097 O.C.T SAKURA Cat# 4583 DAPI Southern Biotech Cat# 0100-20 Mouse Csf3 mRNA probes PinpoRNA Cat# 129851-B1 EGTA Sigma Cat# 3777 Collagenase Sigma Cat# C5138 BSA Solarbio Cat# A8010 PBS Cytiva Hyclone Cat# SH30256.01 Percoll Cytiva Cat# 17-0891-01 HEPES Gibco Cat# 15630080 LT1 Mirus Bio Cat# MIR 2300 Puromycin Thermo Scientific Cat# A11138-03 PEI Polysciences Cat# 23966-100 Lipofectamine 3000 Thermo Scientific Cat# L3000015 NP-40 buffer Solarbio Cat# N8032 Protease inhibitor cocktail Roche Cat# 11873580001 PMSF Solarbio Cat# P0100 Anti-HA magnetic beads MCE Cat# HY-K0201A Flag-beads Genscript Cat# L00432-1 Tween 20 Sigma Cat# P1379 RIPA Solarbio Cat# R0020 Non-fat milk BBI Cat# A600669 D-luciferin Cayman Chemical Cat# 25835 Critical commercial assays TRIzol Reagent Thermo Scientific Cat# 15596018 RNA fluorescence in situ hybridization (FISH) kit PinpoRNA Cat# PIF1000 SETDB1 IHC kit Proteintech Cat# KHC0067 ALT Nanjing Jiancheng Bioengineering Institute Cat# C009-2-1 AST Nanjing Jiancheng Bioengineering Institute Cat# C010-2-1 Human albumin ELISA kits Proteintech Cat# KE00076 cDNA Synthesis SuperMix Novoprotein Cat# E047 SYBR qPCR SuperMix plus Novoprotein Cat# E096 Chromatin Profile Kit Novoprotein Cat# N248-01A NovoNGS® CUT&Tag® 3.0 High-Sensitivity Kit Novoprotein Cat# N259-YH01-01B Deposited data PHx-CRISPR screening This paper SRA:PRJNA1055274 RNA-seq This paper SRA:PRJNA1055274 ATAC-seq This paper SRA:PRJNA1055274 CUT&Tag seq This paper SRA:PRJNA1055274 70% PHx RNA-seq Nicolas Guex and Winship Herr GEO: GSE95135 Experimental models: Cell lines Cell line: THLE-2 ATCC Cat# CRL-2706; RRID:CVCL_3803 (Continued on next page) 16 Cell Reports 44, 115843, June 24, 2025
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